Phenotyping of FOXP3⁺ Tregs.

<p><b>A</b>) Gating strategy for accurate determination of the proportion of regulatory T cells in PBMCs 1. Lymphocytes; 2. CD4⁺ T cells; 3. CD4⁺CD45RA⁺FOXP3^low T cells; 4. CD4⁺CD45RA^-FOXP3^low T cells; 5.CD4⁺CD45RA^-FOXP3^high T cells); <b>B</b>) Percentage of blood CD45RA^-FOXP3^low non-Tregs (gray bars), CD45RA^-FOXP3^high T cells (black bars) and CD45RA⁺FOXP3^low T cells (white bars) among the blood CD4⁺ T cells of infected patients (group 1; <i>n</i> = 76); <b>C</b>) Proportion of CD45RA^-FOXP3^high T cells and CD45RA⁺FOXP3^low T cells expressing CD25, CCR7, CXCR3, CCR5, or CTLA-4 among blood CD4⁺ T cells in 45 patients (excluding splenectomized patients). A nonparametric test was used. * <i>p</i><0.01.