Imaging of 650 nm fluorophore-conjugated IGF-1R antibody targeting of subcutaneous colon tumors in vivo.

<p>(A) The mouse that is imaged under both white light and fluorescence illumination. The intensity of fluorescence from the HCT 116 and HT-29 subcutaneous tumors is much greater than background. (B) Hematoxylin & eosin staining (x200, left). Fluorescence immunostaining for IGF-1R (x20 regular objective, IV-100 scanning laser microscope (Olympus) with a 633 nm laser, right) of frozen HCT 116 tumor samples shows fluorescence on the membrane of the cancer cells.