Inflammatory and anti-inflammatory macrophage markers by flow cytometry in kidneys from aldosterone treated rats.

<p>(A) Representative dot-plots of the CD45 and CD68 staining in cells gated in the low SSC/FSC window (A, left) from kidney suspensions in control (Control), spironolactone treated animals (Spiro), aldosterone+salt-treated animals (Aldo) and aldolsterone+salt plus spironolactone treated animals (Aldo+Spiro). The box inside the dot plots identify cell population expressing CD45 and CD68. Cells included in this box are analysed in down dot plots for CD86 and CD163 expression. Dot plot for peritoneal macrophages as positive control for the detection of CD45, CD68 (A, right). The histogram represent the CD163 expression in CD45⁺/CD68⁺ cells. The vertical dotted line show the isotype control. (B) The bar chart represents the relative number of CD68⁺ cells in kidneys from control (Control), spironolactone treated animals (Spiro), aldosterone+salt-treated animals (Aldo) and aldolsterone+salt plus spironolactone treated animals (Aldo+Spiro). The data are the means ± SEM, n = 2. (C) Graph represents the relative number of CD86 positive cells on CD68 population in control (Control), spironolactone treated animals (Spiro), aldosterone+salt-treated animals (Aldo) and aldolsterone+salt plus spironolactone treated animals (Aldo+Spiro).The data are the means ± SEM, n = 2.