Determination of the specificity of MoAb 4E3 with CE from <i>F</i>. <i>gigantica</i>, other trematode, cestode and nematode parasites by using indirect ELISA and immunoblot analysis.

<p>(A) ELISA OD values of cross-reactivities of MoAb 4E3 and antigens from various trematode, cestode and nematode species. Fg = <i>F</i>. <i>gigantica</i>, Fh = <i>F</i>. <i>hepatica</i>, Ge = <i>G</i>. <i>explanatum</i>, Ep = <i>E</i>. <i>pancreaticum</i>, Pc = <i>P</i>. <i>cervi</i>, Pg = <i>P</i>. <i>gracile</i>, Cc = <i>C</i>. <i>cotylophorum</i>, Fc = <i>F</i>. <i>cobboldi</i>, Gc = <i>G</i>. <i>crumenifer</i>, Ss = <i>S</i>. <i>spindale</i>, Mb = <i>M</i>. <i>benedeni</i>, Ac = <i>A</i>. <i>centripunctata</i>, Ts = <i>Trichuris</i> sp., Hp = <i>H</i>. <i>placei</i> and Sp = <i>S</i>. <i>labiato-papillosa</i>, Sm = <i>S</i>. <i>mansoni</i>, Sme = <i>S</i>. <i>mekongi</i>, Sj = <i>S</i>. <i>japonicum</i>, and Ov = <i>O</i>. <i>viverrini</i>. Undil = undiluted MoAb hybridoma fluid, MoAb dilution = MoAb hybridoma fluid diluted at 1:10, 1: 100, 1: 500 and 1: 1,000. The cut-off value is indicated by a horizontal dashed line and calculated as the mean OD of negative controls plus 3 SD. The OD values greater than this cut-off value are considered to be positive. (B) Immunoblotting detection of the cross-reactivities of MoAb 4E3 with CE from <i>F</i>. <i>gigantica</i> and other trematode parasites. Fg = <i>F</i>. <i>gigantica</i> (lane 2), Fh = <i>F</i>. <i>hepatica</i> (lane 3), Ge = <i>G</i>. <i>explanatum</i> (lane 4), Ep = <i>E</i>. <i>pancreaticum</i> (lane 5), Pc = <i>P</i>. <i>cervi</i> (lane 6), Pg = <i>P</i>. <i>gracile</i> (lane 7), Cc = <i>C</i>. <i>cotylophorum</i> (lane 8), Fc = <i>F</i>. <i>cobboldi</i> (lane 9) and Gc = <i>G</i>. <i>crumenifer</i> (lane 10) and Ss = <i>S</i>. <i>spindale</i> (lane 11), (C) Immunoblotting detection of the cross-reactivities of MoAb 4E3 with CE from <i>F</i>. <i>gigantica</i>, other trematode, cestode and nematode parasites. Mb = <i>M</i>. <i>benedeni</i> (lane 3), Ac = <i>A</i>. <i>centripunctata</i> (lane 4), Ts = <i>Trichuris</i> sp. (lane 5), Hp = <i>H</i>. <i>placei</i> (lane 6), Sp = <i>S</i>. <i>labiato-papillosa</i> (lane 7), Sm = <i>S</i>. <i>mansoni</i> (lane 8), Sme = <i>S</i>. <i>mekongi</i> (lane 9), Sj = <i>S</i>. <i>japonicum</i> (lane 10) and Ov = <i>O</i>. <i>viverrini</i> (lane 11). Lane 1 of B and C is CE from <i>F</i>. <i>gigantica</i> blotted with the myeloma culture fluid (MCF), which is used as the negative control. STD is the lane containing standard protein molecular weight markers indicated on the left side.