Structural based comparison of residue F258 in various polyomavirus T-ag OBDs.

<p><b>A.</b> (Left). Superposition of the apo structures of the JCV T-ag OBDs [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005362#ppat.1005362.ref042" target="_blank">42</a>], including the structure of the JCV T-ag OBD F258L mutant (F258 in green, L258 in orange) indicating the position of residue 258. (Right). Superposition of all of the apo structures of the JCV and SV40 T-ag OBDs indicating the positions of JCV T-ag residue F258 and SV40 T-ag residue F257 (colored as above). <b>B.</b> (Left). Superposition of the DNA bound forms of the SV40 OBD [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005362#ppat.1005362.ref027" target="_blank">27</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005362#ppat.1005362.ref028" target="_blank">28</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005362#ppat.1005362.ref035" target="_blank">35</a>], showing the location of T-ag residue F257 in magenta. (Right). The location of SV40 T-ag OBD residue F257 (magenta) in the T-ag_131-627 crystal structure [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005362#ppat.1005362.ref031" target="_blank">31</a>]. <b>C.</b> Superposition of all of the above SV40 and JCV T-ag structures showing the relative locations of F257/258 (colored as above). The insert depicts a close up of the three main orientations of residue 258 (257 in SV40); the central "apo position" and the two distal orientations (the distance seperating the distal orientations is indicated (~ 13 Å). PDB IDs used in these analyses were: 1) JCV T-ag OBD apo (4NBP, 4LIF, 4LMD), 2) SV40 T-ag OBD apo (2FUF, 2IF9, 2IPR, 2ITJ, 3QK2), 3) SV40 T-ag OBD co-structures with DNA (2ITL, 2NL8, 2NTC, 4FGN, 5D9I) and 4) the SV40 T-ag_131-627 fragment with DNA (4GDF).