Replication of <i>legS2</i> mutant is independent of host cell death.

<p>(A) Wild-type BMDMs were not treated (NT) or infected with the type IV secretion mutant <i>dotA</i>, wild-type <i>L</i>. <i>pneumophila</i>, JR32, or <i>legS2</i> mutant for 24h at MOIs of 0.5 and 5. Then, the fold change in LDH release was measured from the overall population of macrophages. The data represents the mean + SD of n = 3. (B) Wild-type C57BL/6 (B6) were not treated (NT) or infected with wild-type <i>L</i>. <i>pneumophila</i>, JR32, <i>legS2</i>, or <i>dotA</i> mutant for 8 h. Salmonella infection (Sal) was used as a positive control for caspase-3 activation. A Western blot with caspase-3 antibody was used to detect casp-3 activation. β-actin was used as a loading control.