35S:<i>CFLAP1SRDX</i> plants had opposite phenotypes to 35S:<i>CFLAP1</i> plants.

<p>(A) The schematic diagram of 35S:<i>CFLAP1SRDX</i> construct. CaMV35S, CFLAP1 CDS, EAR motif and Nos represent the 35S promoter, <i>CFLAP1</i> coding sequence, the repression domain of 12 amino acid residues, and the <i>NOS</i> terminator sequence, respectively. (B) and (C) Transient effector-reporter expression assay in tobacco leaves, with the <i>CO</i> promoter driving a luciferase gene as a reporter, and the 35S:<i>CFLAP1</i> and 35S:<i>CFLAP1SRDX</i> as effectors. Luminescence imaging is shown 48 hours after co-infiltration with the constructs indicated at left. (D) The result of RT-PCR. From right to left, wild type, transgenic lines 35S:<i>CFLAP1SRDX-1</i>, 35S:<i>CFLAP1SRDX-41</i>, 35S:<i>CFLAP1SRDX-43</i>, and 35S:<i>CFLAP1SRDX-48</i>, respectively.<i>TUB2</i> was used as an internal control. (E) Epicuticular wax components of 35S: <i>CFLAP1SRDX-48</i> and wild-type stems. Numbers indicate the main chain length of each constituent. Each value is the mean + SD of three biological replicates. At least 5 individual stems were used for each replicate. (F) Epicuticular wax components of 35S: <i>CFLAP1SRDX-48</i> and wild-type rosette leaves. Numbers indicate the main chain length of each constituent. Each value is the mean + SD of three biological replicates. At least 7 individual rosette leaves were used for each replicate. Level of significance obtained with a Student’s <i>t</i> test is marked by the following: *, p<0.05; ***, p<0.01.