Dystrophin and spectrin expression in patient and normal muscle.

<p><b>(a)</b> Dystrophin immunofluorescence using Novocastra Laboratories antibodies, NCL DYS-1 (upper row, specific for the rod domain) NCL DYS-2 (upper middle row, specific for the carboxy-terminus) and NCL-DYS 3 (lower middle row, specific for the amino-terminus) and spectrin (lower row) on patient (left panel) and normal human muscle (right panel) cryosections. Nuclei are stained with DAPI. (Bar = 100μm). (b) Western blot using NCL-DYS 1 to detect dystrophin in normal and patient muscle sample. (c) Sequencing of the <i>DMD</i> RT-PCR exon 1–10 amplicon from the exon 5-deletion patient showing the junction of <i>DMD</i> exons 4 and 6.