Intra- and inter-subgroup relationships based on <i>cpn</i>60 and whole genome sequences.

<p>Density plots of pairwise comparisons within and between <i>cpn</i>60 subgroups. A) Plot based on 2,278 comparisons with 68 aligned <i>cpn</i>60 universal target sequences (552 bp). The long tail of the intra-subgroup distribution is explained by low identity of outliers NR010 and JCP8481A with the rest of subgroup A. B) Plot based on ANIm (average nucleotide identity using MUMmer) including 780 comparisons with 40 whole genome sequences. Tail is due to low identity of JCP8481A/B with the rest of subgroup A. C) Plot based on inter-strain correlation coefficient of tetranucleotide signature frequencies (780 comparisons, 40 genomes), with correlation values >0.99 between strains from the same <i>cpn</i>60 subgroup. Small peak at ~0.98 indicates comparisons between JCP8481A/B and other subgroup A genomes. D) Plot based on 16S rRNA gene sequences (231 comparisons with 22 sequences of 1408bp), showing overlap of inter- and intra-subgroup percent identity comparisons for this gene.