Generation of mice encoding a conditional allele of <i>Atoh8</i>.

<p>(A) Schematic representation of the generation of the <i>Atoh8</i> conditional allele construct. Detailed explanation is provided in Methods (B) Representative genotyping PCR amplification of <i>Atoh8</i>^flox/flox wild-type and <i>Atoh8</i>^flox/+. PCR for detection of Cre recombinase is shown on the right; 100 pb molecular marker brightest line corresponds to 500 bp. Floxed allele 400 bp, wild type allele 290 bp; Cre 600 bp (C) Scheme and representative PCR amplification verifying Cre-mediated recombination of the <i>Atoh8</i> floxed allele in the pancreas and not in the liver from Atoh8 Δ^panc embryos at (E)15.5. Recombination mediated by Cre excises <i>Atoh8</i> exon1 that codifies for the 80% of the protein. The PCR amplification of the loxP region (primers 1 and 2) detects a band when recombination occurs; primers 3 and 4 are used as internal amplification control (D) <i>Atoh8</i> mRNA expression levels in (E)14.5 pancreata from Atoh8 Δ^panc and littermate controls as assessed by qRT-PCR. n = 7 per genotype; mean ± SE; ***p < 0.001 vs Control.