Role of NF-κB in the regulation of SEMA7A.

<p><b>A)</b> Graphic representation of the putative SEMA7A promoter. Four potential NF-κB binding elements were identified in the upstream sequence relative to the transcription start site (TSS). <b>B)</b> ChIP assay was employed to examine NF-κB binding to the human SEMA7A promoter in A549 monolayers after exposure to TNF-α. <b>C)</b> A549 cells were transfected with the SEMA7A reporter plasmid and site-directed mutation of each NF-κB binding site was performed. Empty reporter vector (pGL4.17) was used as a negative control and SEMA7A full length (SEMA7A-NF-κB_FL) represents the SEMA7A promoter construct without site-directed mutation. All data are expressed as mean±SEM compared to SEMA7A-NF-κB_FL, ***<i>P</i> < 0.001 as indicated, n = 3/ group, one representative blot of three is demonstrated).