Proinflammatory response by concentration dependent TLR9 activation in acute vascular injury.

<p>WT mice were subjected to an electric denudation of the left carotid artery as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146326#pone.0146326.g001" target="_blank">Fig 1</a> and received repetitive s.c. injections of 0.625nM (n = 7), 6.25nM (n = 7), 18nM (n = 6), 30nM CpG ODN (n = 7) or vehicle (n = 7) over 7 days. Splenic CD3 positive lymphocytes from 18nM and 30nM CpG ODN treated mice expressed more IFN-γ and RANTES compared to vehicle controls (a). TLR9 activation by 6.25nM, 18nM and 30nM CpG ODN significantly impaired reendothelialization in WT mice compared to vehicle controls (b). Further, stimulation with 18nM and 30nM CpG ODN elevated levels of circulating CD11b positive cells (c). Data are presented as mean±SEM. Statistical analysis was performed using ANOVA (Holm-Sidak's multiple comparisons test). *p<0.05, **p<0.001, p***<0.0001.