Differential distribution of parasites in terms of their shape- and appearance-based phenotypes as demonstrated by clustering.

<p>To enable visualization, the clustering results from the six-dimensional principal component analysis (PCA) space are mapped to a two-dimensional PCA space where the X-axis corresponds to the first PCA component and the Y-axis corresponds to the second PCA component. Control parasites are shown in blue. Parasites that were co-clustered with the controls but had been exposed to compounds are shown in green. These parasites did not exhibit significant phenotypic changes as a result of compound exposure and were consequently very similar to the control parasites. Parasites that had been exposed to compound and exhibited significant changes in their phenotypes are shown in red. A small number of control parasites (blue coloured “+” symbols) exhibited naturally occurring degeneracies and were placed outside the control cluster by the algorithm. Both the differential distribution and separability of the phenotypes exhibited by the control parasites from those exhibited by the treated parasites are obvious.