In vitro analysis of Dnajb8 KO cells.

<p>(A) Representative images of WT cells and Dnajb8 KO cells. Bars indicate 100 μm. (B) Cell proliferation assay of WT cells and Dnajb8 KO cells. 10⁴ cells were cultured and the number of cells was counted at 1, 3 and 5 days. Data represent means ± SD. The difference between WT cells and two Dnajb8 KO cells was examined for statistical significance using Student’s <i>t</i>-test. (C) Representative images of spheres derived from WT cells and Dnajb8 KO cells. Bars indicate 100 μm. (D) Sphere formation assay. To assay sphere formation efficiency, 10³ cells were cultured in floating condition for 22 days and the numbers of spheres were counted under a microscope in all fields. (E) Side population (SP) assay. WT cells and Dnajb8 KO cells were stained with Hoechst 33342 dye with or without verapamil and analyzed using a FACSAria II cell sorter.