Sample VSV-rWT viral and innate immune reporter protein expression kinetics from six individual cells.

<p>Kinetic parameters have been extracted from the trajectories of both the viral (red) and host (green) trajectories and displayed in the top left corner of each figure. Maximum yields from individual cells vary greatly (a-b), host reporter expression can be greater or lesser than viral gene expression given similar starting conditions (c-d), and cells can lyse (d) or remain intact during imaging. Single-cell analysis can detect and quantify cells displaying rare behavior, such as those that appear to have a basal level of innate immune activation all the time (e) and cells infected with wt-VSV that express ZS-Green despite encoding a functional matrix gene.