Analysis of anti-HCV potency and RNAi activity of various siRNAs targeting the IRES subdomain IIIf.

<p>(A) Passenger strand sequences of siIE22 and six siRNAs sharing their targets with siIE22 in the IRES subdomain IIIf. (B) Evaluation of anti-HCV efficacy of a set of selected siRNAs (100 pM each) using an HCV replicon expressing an Rluc reporter. Luciferase activity at 48 h post-siRNA treatment is shown. Sc, scrambled siRNA. (C) Antiviral efficacy of gs_PS1 siIE22 and siIE318_27 in R-1 cells harboring an HCV subgenomic replicon. (D) <i>In vitro</i> target cleavage assays were performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146710#pone.0146710.g002" target="_blank">Fig 2A</a>. P, 5′ ³²P-radiolabeled 31-nt long HCV IRES probe; CP, cleaved probe. (E) Comparison of antiviral activity of indicated siRNAs targeting the region shared with siIE22 in R-1 cells. In (B), (C), and (E), *, <i>P <</i> 0.05; **, <i>P <</i> 0.01.