Parasite burden 9 months after challenge.

<p>Hamsters were challenged in the left ear with virulent <i>L</i>. <i>donovani</i> and presence of parasites was detected 9 months after challenge. The draining lymph node (A), spleen (B) and liver (C) were used in limiting dilution assay and data expressed as number of parasites/organ. Statistical differences (p<0.05) are indicated in letters (a: G1, b: G2 and c: G3). (D) Comparison of spleen size 9 months after challenge. The median of spleen size of hamsters of G1, G2, G3 and G4 was measured in centimeter. A representative sample from 6 immunized and challenged hamsters is shown.