Phosphorylation of Foxo1 is regulated by PI3K, p38 and JNK.

<p>(A) HepG2 cells were pre-treated with 50 μM SB202190 (p38 inhibitor) or SP600125 (JNK inhibitor) for 1 h; (B) Cells were pre-treated with 50 μM LY294002 (PI3K inhibitor) or 2 μM Akt IV (Akt inhibitor) for 1 h; (C) Cells were transfected with 50 nM control or Rictor siRNA. Cells from (A)—(C) were further treated with 5 μM Cd for 1 h then harvested. Cell extracts were prepared for immunoblotting and quantification of c-Myc, Foxo1, phospho-Foxo1 [p-Foxo1(T24)], Akt and Akt phosphorylated at Ser473 (p-Akt). Numbers underneath the Western blotting represent the amount of phospho-Foxo1 relative to that of the loading control (actin).