Antioxidative stress reaction and gene expression.

<p>The level of anti-oxidative stress(A) and lipid peroxidation(B) at 3 days after myocardium reperfusion (n = 4). RIPoC+IPoC, RIPoC and IPoC procedure increased the levels of anti-oxidative stress and decreased the lipid peroxidation reaction in reperfusion myocardium (p<0.01).The SOD1 and SOD2 gene expression level at the borderline of ischemic myocardium were detected(C). The gene expression of SOD1 and SOD2 were enhanced in RIPoC+IPoC, RIPoC and IPoC group compared with that in IR group (p<0.01). Group A: RIPoC+IPoC group, received the combined algorithms of RIPoC and IPoC; Group B: RIPoC group, which received 3 cycles of 30 seconds reperfusion and re-occlusion on the limb at the onset of reperfusion; Group C: IPoC group, received 3 cycles of 30 seconds reperfusion and re-occlusion on the LAD; Group D: IR group, received reperfusion without interruption after ischemia; Group E: sham group, received only open chest.