An immunochromatographic (IC) strip test is developed for diagnosis of fasciolosis by <i>F</i>. <i>gigantica</i>: Experiment trial.

<p>(A) A schematic diagram of the immunochromatographic (IC) strip test showing several components: a sample pad, a conjugate pad, an immobilized nitrocellulose membrane (control and test antibody) and an absorbent pad. (B) The samples of the IC strip test for deciding the results: a positive result shows two red dots at the test and control regions, while a negative result exhibits only one red dot in the control region. The strip tests are invalid when there is no red dot at the control region. (C-E) Sensitivity testing of the IC strip was studied using a series of dilutions in a buffer (0.0512–20,000 ng/ml) of adult <i>F</i>. <i>gigantica</i> recombinant cathepsin L1 (rCatL1) (C), crude extract (CE) (D), and excretory-secretory (ES) antigens (E). 1 = 20,000 ng/ml, 2 = 4,000 ng/ml, 3 = 800 ng/ml, 4 = 160 ng/ml, 5 = 32 ng/ml, 6 = 6.4 ng/ml, 7 = 1.2 ng/ml, 8 = 0.256 ng/ml, 9 = 0.0512 ng/ml, and 10 = buffer control. (F) The specificity of the IC strips were tested against CE from <i>F</i>. <i>gigantica</i>, other trematode, cestode and nematode parasites. All antigens were tested at 1,000 μg/ml. Fg = <i>F</i>. <i>gigantica</i>, Bf = buffer control, Fh = <i>F</i>. <i>hepatica</i>, Ge = <i>G</i>. <i>explanatum</i>, Ep = <i>E</i>. <i>pancreaticum</i>, Pc = <i>P</i>. <i>cervi</i>, Pg = <i>P</i>. <i>gracile</i>, Cc = <i>C</i>. <i>cotylophorum</i>, Fc = <i>F</i>. <i>cobboldi</i>, Gc = <i>G</i>. <i>crumenifer</i>, Ss = <i>S</i>. <i>spindale</i>, Mb = <i>M</i>. <i>benedeni</i>, Ac = <i>A</i>. <i>centripunctata</i>, Ts = <i>Trichuris</i> sp., Hp = <i>H</i>. <i>placei</i>, Sp = <i>S</i>. <i>labiato-papillosa</i>, Sm = <i>S</i>. <i>mansoni</i>, Sme = <i>S</i>. <i>mekongi</i>, Sj = <i>S</i>. <i>japonicum</i> and Ov = <i>O</i>. <i>viverrini</i>.