Additional file 3: Figure S3. of Directly converted patient-specific induced neurons mirror the neuropathology of FUS with disrupted nuclear localization in amyotrophic lateral sclerosis

FUS is distributed in the nucleus in ALS-FUS patient postcentral gyrus and dorsal horn. (a) DAB staining depicts predominant nucleus localization of FUS (as indicated by their morphology) in the postcentral gyrus of a normal control (CTL 4, top), sporadic ALS patient (middle), and FUS (p.G504Wfs*12) patient (bottom). The enlarged images are shown in the right panels. Scale bars = 10 μm. (b) The dorsal horn of the spinal cord sections from normal control (top), sporadic ALS patient (top), and FUS (p.G504Wfs*12) patient (bottom) were compared. The same predominant nucleus staining of FUS were observed by DAB staining in the dorsal horn neurons (as indicated by their morphology) of a normal control (top), sporadic ALS patient (middle), and FUS (p.G504Wfs*12) patient (bottom). Scale bars = 10 μm. (PDF 378 kb