Additional file 5: Figure S4. of p53 deficiency linked to B cell translocation gene 2 (BTG2) loss enhances metastatic potential by promoting tumor growth in primary and metastatic sites in patient-derived xenograft (PDX) models of triple-negative breast cancer

CTC number correlates with mammary tumor size. Mouse mammary fat pads were engrafted with BC3-p53WT or BC3-p53KD. Whole blood was extracted from mice in a terminal blood draw by cardiac puncture. Red blood cells were lysed, and circulating tumor cells (CTCs) were assessed by flow cytometry for mCherry-positive cells. BLI was performed on mammary tumors and lungs at necropsy. (A and B) CTCs were quantified by flow cytometry and normalized to total photon flux (A) or volume (B) of each corresponding mammary tumor at indicated time points. Error bars represent standard error of the mean (SEM). (C) CTC number was plotted versus photon flux of the mammary tumor within each mouse. Pearson correlation = 0.82, p <0.001. (D) CTC number was plotted versus tumor volume within each mouse. Pearson correlation = 0.61, p = 0.001. (E) CTC number was plotted versus photon flux in the lung. Pearson correlation = 0.79, p