Schematic representation of mRNA splice sites and putative protein topology of the Ca_v1.1 channel T cell variants.

<p>(A) Five new Exons were detected by 5’-RACE (A-E). The ATG of Ca_v1.1 open reading frame is located in Exon C. Exons C-E replace the original Exons 1 and 2 (NM_000069). Alternative splice out of Exon 29 leads to the deletion of the IVS3-S4 linker. Exons encoding transmembrane segments are written below their respective boxes. Introns are represented as lines in between exon boxes. Below is a diagram of putative channel topology of the Ca_v1.1 channel variant. (B) Diagram of putative channel topology of the Ca_v1.1 variant. Transmembrane segments are white boxes, except the S4 voltage sensor domain, which is shown in gray. Sequence comparison between muscle and T cell Ca_v1.1 at the IVS3-S4 linker region is shown.