SIRT1 does not co-localize with HTT inclusions and is aberrantly phosphorylated in HD mice.

<p>(A) Representative immunofluorescence image of isolated nuclei extracted from half brains from R6/2 mice at 14 weeks of age and <i>Hdh</i>Q150 homozygotes at 22 months immunostained for both SIRT1 and mutant HTT (EM48), and counterstained with DAPI. (B) Representative western blot showing SIRT1 levels in half brains from R6/2 mice at 14 weeks of age and <i>Hdh</i>Q150 homozygotes at 22 months as compared to their WT littermates and relative quantification. (C) Western blots of SIRT1 and HTT after SIRT1 immunoprecipitation from half brain lysates of R6/2 and <i>Hdh</i>Q150 homozygotes as compared to their WT littermates. IP = immunoprecipitation; WB = western blotting. <i>Hdh</i> = <i>Hdh</i>Q150 homozygotes. Scale bar, 10 μm.