Inhibition of mTOR reduces cell growth in NRAS mutant cell lines.

<p>A) CHP-212 and SK-N-AS cells were treated with 5nM of the mTOR inhibitors Everolimus or 250nM AZD8055 for 1 hour. Then, cells were lysed and analysed by Western blot. B) All indicated cell lines were left untreated or treated with indicated concentrations of Everolimus for 96 hours. Next, cell growth was measured by Cell Titer Glo according to the manufacturer’s instructions C) Same as B) but the mTOR inhibitor AZD8055 was used instead. D) NRAS mutant and wild-type cell lines were incubated with indicated concentrations of Everolimus for 72 hours. Then, apoptosis was determined by Annexin V and PI staining.