Expression of <i>C</i>. <i>burnetii</i> T4BSS-translocated effectors in mammalian HeLa cells.

<p>(A) Immunofluorescence confocal images show the representative subcellular localization of the indicated GFP- or HA-tagged <i>C</i>. <i>burnetii</i> effector proteins (green) after ectopic expression in HeLa cells. For contrast, DAPI, Mitotracker, or anti-tubulin, anti-calnexin and anti-LAMP-1 antibodies, were used as marked. Scale bars represent 10mm. (B) Expression of the indicated GFP-tagged <i>C</i>. <i>burnetii</i> effectors was analyzed by immunoblot analysis. Protein extracts from transfected HeLa cells were separated by SDS-PAGE, transferred to PVDF membrane and probed with anti-GFP antibody and anti-tubulin as loading control. A representative immunoblot is shown out of three independent experiments with similar results.