Effect of different MWs of HA on the interaction of MSCs and Th cells.

<p>Proliferation of Th cells, in the presence or absence of MSCs, without (A), and with IFNγ supplementation (B). (C) IL10, IFNγ and IL2 secreted levels in medium from activated Th cells with or without MSCs, and with or without IFNγ supplementation. (D) Ratios of secreted IL10/IFNγ, IL10/IL2. Solid filled bars stand for hHA; hatched bars, 150 kDa HA; dotted bars, 7.5 kDa HA and empty bars, no HA. Black color, normal conditions and gray color with red frame, IFNγ-supplemented conditions. All data shown is from one representative MSC donor; additional data from three other donors shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0147868#pone.0147868.s008" target="_blank">S7 Fig</a>. Statistical differences in the presence of MSCs account for MSCs from 4 donors, each tested in triplicate. Statistical differences in the absence of MSCs account for Th cells from one donor tested in quadruplicate. Error bars represent 95% CI. * p<0.05, ** p<0.01 and ***<0.001.