Paf1C subunits, except for Rtf1, are associated in mouse muscle cells but differentially impact histone modifications.

<p><b>(A)</b> Western blot analysis of expression of Paf1C subunits in myoblasts (MB) and myotubes (MT). Rtf1 and Ctr9 are detectable in the chromatin-bound fraction but not whole cell extracts, as indicated. <b>(B)</b> Co-immunoprecipitation of endogenous Paf1C subunits, except for Rtf1, in the chromatin-bound fraction. IgG, control antibody. <b>(C)</b> siRNA-mediated depletion of the indicated Paf1C subunits in myoblasts have distinct effects on the levels of histone marks. siCtrl, non-specific control siRNA. <b>(D)</b> qChIP analysis of the enrichment of the Paf1C at specific loci in myoblasts and myotubes. The number shown for each locus indicates the midway point within the amplicon in base-pairs from the TSS of a specific gene. Gene desert is a genomic region devoid of genes.