Amniotic membrane extract cytotoxicity measurement using the MTT assay.

Effect of protectin on IMR-32 cells.

Sailaja Polavarapu (678607)
Arul M. Mani (678608)
Naveen K. V. Gundala (678609)
Anasuya D. Hari (678610)
Siresha Bathina (678611)
Undurti N. Das (678612)

December 2014

IMR-32 cells were exposed to different doses (1, 5, 10 ng/ml) of protectin (19,20-DiHDPA, 10(S),1...

Cytotoxicity of FA on IEC-6 cells. Effects of FA on viability of IEC-6 cells was measured using the MTT assay.

Shasha He (833918)
Fenghua Liu (296600)
Lei Xu (78676)
Peng Yin (46588)
Deyin Li (2136427)
Chen Mei (2136424)
Linshu Jiang (2136421)
Yunfei Ma (1777753)
Jianqin Xu (833917)

February 2016

Cells were incubated with FA concentrations ranging from 0 to 500 μM for 48 h. Data are presented...

Cell viability detected by MTT assays.

Shengming Zhu (817526)
Jiangang Wang (348255)
Bingkun Xie (817527)
Zhiguo Luo (817528)
Xiukun Lin (233022)
D. Joshua Liao (140193)

October 2015

A: The optical density of MTT is often higher in the cells at 39°C than at 37°C, even when...

Amniotic membrane extract cytotoxicity measurement using real-time cell analysis.

Jan Adamowicz (622230)
Marta Pokrywczyńska (622181)
Jakub Tworkiewicz (845765)
Tomasz Kowalczyk (622178)
Shane V. van Breda (845766)
Dominik Tyloch (845767)
Tomasz Kloskowski (622176)
Magda Bodnar (845768)
Joanna Skopinska-Wisniewska (845769)
Andrzej Marszałek (622180)
Malgorzata Frontczak-Baniewicz (622238)
Tomasz A. Kowalewski (622183)
Tomasz Drewa (622185)

January 2016

Each result was presented as mean from 5 independent experiment with SD bars. No statistically si...

MTT assay to evaluate the cytotoxicity of the compounds towards Raw264.7 cells.

Hai-Jing Zhong (118092)
Li-Juan Liu (293637)
Cheong-Meng Chong (517812)
Lihua Lu (476939)
Modi Wang (393693)
Daniel Shiu-Hin Chan (118095)
Philip Wai Hong Chan (545653)
Simon Ming-Yuen Lee (545654)
Dik-Lung Ma (103041)
Chung-Hang Leung (118097)

April 2014

Cells were incubated with the indicated concentrations of compounds for 18λ = 570 nm after the ad...

Cytotoxicity tests of compounds by MTT assay.

Xuewei Yang (770874)
Shuang Yang (64650)
Hongyu Chai (788005)
Zhaogang Yang (788006)
Robert J. Lee (299150)
Weiwei Liao (788007)
Lesheng Teng (788008)

August 2015

Cells were treated with various compounds for 4h, and grown for another 20h. (A)IC50 of HeLa and ...

Cytotoxic effects of DOX and LMB on A549 cells.

Chuanwen Lu (159114)
Changxia Shao (179012)
Everardo Cobos (7263)
Kamaleshwar P. Singh (179018)
Weimin Gao (17190)

March 2012

A, Cytotoxic effects of DOX alone and DOX+LMB on cell viability of A549 cells as determined by th...

Effect of Am on cell viability.

Bastien Herman (386722)
Aldrian Gudrun (386723)
Anatoly I. Potopalsky (386724)
Jadwiga Chroboczek (134668)
Sergey O. Tcherniuk (386725)

March 2013

(A) HeLa cells were treated with Am for 72 h as described in Material and Methods and cell viabil...

Assessment of Cell health by MTT Assay.

Sanica C. Sakharwade (464146)
Praveen K. Sharma (366724)
Arunika Mukhopadhaya (464147)

September 2013

THP-1 human monocytes were plated and treated with OmpU or protein-buffer or PmB and incubated fo...

Viability and cytotoxicity upon MK-4 incubation for 72 h.

Sissel Beate Rønning (4339753)
Mona Elisabeth Pedersen (4339756)
Ragnhild Stenberg Berg (5059520)
Bente Kirkhus (3937688)
Rune Rødbotten (5059517)

April 2018

A) Bars show the viability (amount of ATP present) of MK-4 treated cells compared to control cell...

MTT assay to determine the changes in residual cell viability in the 2 mM SS pre-treated GSC and SNB19 cells after transfection with 25, 50, and 100 nM miR-30e for 12 h or treatment with 50, 100, and 150 μM PAC for 24 h, or their combinations.

Mrinmay Chakrabarti (284359)
Daniel J. Klionsky (53548)
Swapan K. Ray (284361)

July 2016

After the treatments and incubation, alterations in cell viability were measured by the MTT assay...

Cell viability and cytotoxicity grade for freshly mixed materials in MTT assay (n = 5).

Yao-Zhong Chen (1393603)
Xiao-Ying Lü (4839723)
Gen-Di Liu (4839726)

February 2018

Cell viability and cytotoxicity grade for freshly mixed materials in MTT assay (n = 5).</p

The MTT Assay: Utility, Limitations, Pitfalls, and Interpretation in Bulk and Single-Cell Analysis

Mahshid Ghasemi
Tyron Turnbull
Sonia Sebastian
Ivan Kempson

November 2021

The MTT assay for cellular metabolic activity is almost ubiquitous to studies of cell toxicity; howe...

Comparison of the cytotoxicity increment in MCTS cultures assessed by MTT and LDH release assays.

Wan Yong Ho (308639)
Swee Keong Yeap (298365)
Chai Ling Ho (308641)
Raha Abdul Rahim (308644)
Noorjahan Banu Alitheen (298363)

February 2013

Relative to the untreated control, which was normalized to 0% in both assays, reduction in cell v...

Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study

Mamede, Ana Catarina Manjolinha
Laranjo, Marta
Carvalho, Maria J
Abrantes, Ana M
Pires, A S
Brito, A F
Moura, P
Maia, C J
Botelho, M F

April 2014

Human amniotic membrane (hAM) has recently drawn attention as an upcoming anti-cancer therapy. Regar...

Effect of protectin on IMR-32 cells.

Sailaja Polavarapu (678607)
Arul M. Mani (678608)
Naveen K. V. Gundala (678609)
Anasuya D. Hari (678610)
Siresha Bathina (678611)
Undurti N. Das (678612)

December 2014

IMR-32 cells were exposed to different doses (1, 5, 10 ng/ml) of protectin (19,20-DiHDPA, 10(S),1...

Cytotoxicity of FA on IEC-6 cells. Effects of FA on viability of IEC-6 cells was measured using the MTT assay.

Shasha He (833918)
Fenghua Liu (296600)
Lei Xu (78676)
Peng Yin (46588)
Deyin Li (2136427)
Chen Mei (2136424)
Linshu Jiang (2136421)
Yunfei Ma (1777753)
Jianqin Xu (833917)

February 2016

Cells were incubated with FA concentrations ranging from 0 to 500 μM for 48 h. Data are presented...

Cell viability detected by MTT assays.

Shengming Zhu (817526)
Jiangang Wang (348255)
Bingkun Xie (817527)
Zhiguo Luo (817528)
Xiukun Lin (233022)
D. Joshua Liao (140193)

October 2015

A: The optical density of MTT is often higher in the cells at 39°C than at 37°C, even when...

Amniotic membrane extract cytotoxicity measurement using real-time cell analysis.

Jan Adamowicz (622230)
Marta Pokrywczyńska (622181)
Jakub Tworkiewicz (845765)
Tomasz Kowalczyk (622178)
Shane V. van Breda (845766)
Dominik Tyloch (845767)
Tomasz Kloskowski (622176)
Magda Bodnar (845768)
Joanna Skopinska-Wisniewska (845769)
Andrzej Marszałek (622180)
Malgorzata Frontczak-Baniewicz (622238)
Tomasz A. Kowalewski (622183)
Tomasz Drewa (622185)

January 2016

Each result was presented as mean from 5 independent experiment with SD bars. No statistically si...

MTT assay to evaluate the cytotoxicity of the compounds towards Raw264.7 cells.

Hai-Jing Zhong (118092)
Li-Juan Liu (293637)
Cheong-Meng Chong (517812)
Lihua Lu (476939)
Modi Wang (393693)
Daniel Shiu-Hin Chan (118095)
Philip Wai Hong Chan (545653)
Simon Ming-Yuen Lee (545654)
Dik-Lung Ma (103041)
Chung-Hang Leung (118097)

April 2014

Cells were incubated with the indicated concentrations of compounds for 18λ = 570 nm after the ad...

Cytotoxicity tests of compounds by MTT assay.

Xuewei Yang (770874)
Shuang Yang (64650)
Hongyu Chai (788005)
Zhaogang Yang (788006)
Robert J. Lee (299150)
Weiwei Liao (788007)
Lesheng Teng (788008)

August 2015

Cells were treated with various compounds for 4h, and grown for another 20h. (A)IC50 of HeLa and ...

Cytotoxic effects of DOX and LMB on A549 cells.

Chuanwen Lu (159114)
Changxia Shao (179012)
Everardo Cobos (7263)
Kamaleshwar P. Singh (179018)
Weimin Gao (17190)

March 2012

A, Cytotoxic effects of DOX alone and DOX+LMB on cell viability of A549 cells as determined by th...

Effect of Am on cell viability.

Bastien Herman (386722)
Aldrian Gudrun (386723)
Anatoly I. Potopalsky (386724)
Jadwiga Chroboczek (134668)
Sergey O. Tcherniuk (386725)

March 2013

(A) HeLa cells were treated with Am for 72 h as described in Material and Methods and cell viabil...

Assessment of Cell health by MTT Assay.

Sanica C. Sakharwade (464146)
Praveen K. Sharma (366724)
Arunika Mukhopadhaya (464147)

September 2013

THP-1 human monocytes were plated and treated with OmpU or protein-buffer or PmB and incubated fo...

Viability and cytotoxicity upon MK-4 incubation for 72 h.

Sissel Beate Rønning (4339753)
Mona Elisabeth Pedersen (4339756)
Ragnhild Stenberg Berg (5059520)
Bente Kirkhus (3937688)
Rune Rødbotten (5059517)

April 2018

A) Bars show the viability (amount of ATP present) of MK-4 treated cells compared to control cell...

MTT assay to determine the changes in residual cell viability in the 2 mM SS pre-treated GSC and SNB19 cells after transfection with 25, 50, and 100 nM miR-30e for 12 h or treatment with 50, 100, and 150 μM PAC for 24 h, or their combinations.

Mrinmay Chakrabarti (284359)
Daniel J. Klionsky (53548)
Swapan K. Ray (284361)

July 2016

After the treatments and incubation, alterations in cell viability were measured by the MTT assay...

Cell viability and cytotoxicity grade for freshly mixed materials in MTT assay (n = 5).

Yao-Zhong Chen (1393603)
Xiao-Ying Lü (4839723)
Gen-Di Liu (4839726)

February 2018

Cell viability and cytotoxicity grade for freshly mixed materials in MTT assay (n = 5).</p

The MTT Assay: Utility, Limitations, Pitfalls, and Interpretation in Bulk and Single-Cell Analysis

Mahshid Ghasemi
Tyron Turnbull
Sonia Sebastian
Ivan Kempson

November 2021

The MTT assay for cellular metabolic activity is almost ubiquitous to studies of cell toxicity; howe...

Comparison of the cytotoxicity increment in MCTS cultures assessed by MTT and LDH release assays.

Wan Yong Ho (308639)
Swee Keong Yeap (298365)
Chai Ling Ho (308641)
Raha Abdul Rahim (308644)
Noorjahan Banu Alitheen (298363)

February 2013

Relative to the untreated control, which was normalized to 0% in both assays, reduction in cell v...

Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study

Mamede, Ana Catarina Manjolinha
Laranjo, Marta
Carvalho, Maria J
Abrantes, Ana M
Pires, A S
Brito, A F
Moura, P
Maia, C J
Botelho, M F

April 2014

Human amniotic membrane (hAM) has recently drawn attention as an upcoming anti-cancer therapy. Regar...

Effect of protectin on IMR-32 cells.

Sailaja Polavarapu (678607)
Arul M. Mani (678608)
Naveen K. V. Gundala (678609)
Anasuya D. Hari (678610)
Siresha Bathina (678611)
Undurti N. Das (678612)

December 2014

IMR-32 cells were exposed to different doses (1, 5, 10 ng/ml) of protectin (19,20-DiHDPA, 10(S),1...

Cytotoxicity of FA on IEC-6 cells. Effects of FA on viability of IEC-6 cells was measured using the MTT assay.

Shasha He (833918)
Fenghua Liu (296600)
Lei Xu (78676)
Peng Yin (46588)
Deyin Li (2136427)
Chen Mei (2136424)
Linshu Jiang (2136421)
Yunfei Ma (1777753)
Jianqin Xu (833917)

February 2016

Cells were incubated with FA concentrations ranging from 0 to 500 μM for 48 h. Data are presented...

Cell viability detected by MTT assays.

Shengming Zhu (817526)
Jiangang Wang (348255)
Bingkun Xie (817527)
Zhiguo Luo (817528)
Xiukun Lin (233022)
D. Joshua Liao (140193)

October 2015

A: The optical density of MTT is often higher in the cells at 39°C than at 37°C, even when...

Amniotic membrane extract cytotoxicity measurement using the MTT assay.

Abstract

Extracted data

Amniotic membrane extract cytotoxicity measurement using the MTT assay.

Abstract

Extracted data

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