SDS-polyacrylamide gel electrophoresis analysis of the artificial cysteine mutant scFv-C_kappa fusion protein PEG conjugates.

<p>Artificial cysteine-mutant scFv-C_kappa fusion protein PEG conjugates were subjected to 4–12% (w/v) SDS-polyacrylamide gel electrophoresis without the addition of a reducing agent. After electrophoresis, the gel was stained with Coomassie Blue (A) or barium iodide (I₂) (B). An artificial cysteine-mutant scFv-C_kappa fusion protein not conjugated with PEG and the original scFv-C_kappa fusion protein were used as controls.