Effects of <i>HBx</i> silencing in HepG2.2.15 and HBx overexpression in HepG2 on expression of Notch receptors and ligands.

<p>(A) HepG2.2.15 was transiently transfected with siRNA specific for <i>HBx</i> or control scramble siRNA as described in materials and methods. Cell lysates from were analysed by Western blot. β-actin was used as loading control. (B-C) HepG2 was transiently transfected with the control pEGFP vector or pEGFP-HBx for 24 hr. Cells were sorted for GFP⁺ cells. Total RNA from three cell lines were analyzed for mRNA expression of Notch receptors, Notch ligands and <i>Hes1</i> by qPCR. The results are representative of at least two independent experiments where *, ** and *** indicates statistical significance at the <i>p</i> <0.05, <i>p</i><0.01 and <i>p</i><0.001 level, respectively.