Involvement of Rab30 in starvation-induced autophagosome formation.

<p>(A) Confocal microscopic images of mCherry–LC3 puncta with EmGFP–Rab30 in starvation conditions. HeLa cells that expressed mCherry–LC3 and EmGFP–Rab30 were cultured in starvation medium for 2 h and fixed. Yellow arrows indicate Rab30-colocalized LC3 puncta. (B) Confocal microscopic images of EmGFP–LC3 puncta in Rab30 knockdown cells. HeLa cells stably expressing EmGFP–LC3 were transfected with a control siRNA or Rab30 siRNA and cultured in regular medium or starvation medium for 2 h and fixed. Bars, 10 μm. (C) Effect of knockdown of the Rab30 on starvation-induced autophagosome formation. The number of LC3 dots was quantified in confocal microscopic images. The data shown represent the results for >10 images and each percentage represents the mean value ± SD based on three independent experiments. NS, not significant.