Involvement of Rab30 in GAS-induced autophagy is independent of its role in maintaining the structural integrity of the Golgi apparatus.

<p>(A) HeLa cells were transfected with EmGFP-LC3 and siRNAs against the indicated target mRNAs, or were treated with BFA or GA, as indicated. Subsequently, the cells were infected with GAS (MOI = 100). The cells were then fixed, permeabilized, and stained with DAPI. The percentage of cells with GcAVs was quantified. (B) HeLa cells were transfected with siRNAs against indicated target mRNAs, or treated with BFA or GA, after which they were infected with GAS. Cells were fixed, permeabilized, and immunostained with an anti-GM130 antibody. The percentage of cells with intact Golgi structure was quantified. The data shown represent the results of >200 cells in terms of the mean value ± SD from 3 independent experiments. ** <i>P</i> < 0.01.