Pulmonary cytokine expression in WT and RORγt-tg mice after MAC Infection.

<p>The expression of IFN-γ, TNF-α, IL-4, IL-6, IL-10, IL-12p40, IL-17, and IL-23p19 in the lungs of WT and <i>RORγt-tg</i> mice 2 months after the intratracheal inoculation of 1 x 10⁷ CFU of MAC or saline (Cont). The expression of each mRNA was analyzed by qRT-PCR, and the y-axis of each graph represents the relative expression of the respective genes calculated using the ∆∆CT method and normalized against GAPDH mRNA. Experiments were performed in duplicate with five mice in each group. *Significant difference between MAC and Cont group (p<0.05). #Significant difference between genotypes after MAC infection (p<0.05). Data are expressed as the mean ± SEM.