Concentration of RecA protein, as measured by ELISA, in <i>S</i>. <i>enterica ΔcheR</i> cells harboring plasmid pUA1127 (eYFP::<i>cheR</i>) and growing in liquid medium.

<p>Samples were taken periodically after the removal of mitomycin C from cultures pre-treated with 0.08 μg mitomycin C/mL for 300 min (◇, discontinuous dotted line). The amount of RecA of a non-treated culture (□, discontinuous line) and a continuously treated culture (◆, continuous line) served as controls. The RecA concentration is expressed as the number of RecA molecules per μg of total protein. The results are the mean of three independent experiments. Error bars represent the standard deviation. The relative RecA concentration after mitomycin C removal (boxed) was calculated as the mean RecA concentration at each time point with respect to the mean RecA concentration of the non-treated culture [1.13 (±0.25) x 10¹¹ molecules per μg of total protein].