Turnover of aRNAs is influenced by CPT.

<p>Cells where firstly stimulated for antisense accumulation by a 4 hours treatment with CPT (time 0). Successively CPT was removed (black lines) or maintained (red lines) in the medium. In addition, FLV was added to block transcription in absence (green lines) and in presence (blue lines) of CPT, and antisense transcript levels determined by rtqPCR after additional 20, 40 and 60 minutes. PCR determinations were normalized to β-actine mRNA and to aRNAs levels at time 0. A representative experiment is here reported.