Lysate Sensitized T-Cells from MUC1.Tg Mice Conferred Protection in Adoptive Transfer Experiments (Winn Assay).

<p><b>(A)</b> The sorted spleen-derived effector T-cells from MUC1.Tg mice immunized with rotating tumor cell lysates were co-injected with B16.MUC1 tumor cells (T-cell to tumor cell ratio of 10:1) either directly or after stimulation <i>in vitro</i> with DCs pulsed with B16.MUC1 tumor cell lysates. T-cells from non-immunized MUC1.Tg mice were co-injected with B16.MUC1 tumor cells as controls. The mice that received T-cells after <i>in vitro</i> sensitization showed complete protection from tumor growth, (p<0.001). <b>(B)</b> The B16.MUC1 tumors resistant to T-cells from immunized MUC1.Tg mice showed low MUC1 expression vs non-immunized mice (p = 0.02). <b>(C)</b> Corresponding histograms of MUC1 expression are shown. Experiment was repeated two times, n = 4 mice/group.