Pro- and anti-inflammatory cytokine mRNA expression in native CD14⁺ monocytes.

<p>Unstimulated adult CD14⁺ monocytes were exposed to 100μg/ml Curosurf, 100μg/ml POPG, 100μg/ml PLs, 100μg/ml PLs+SP-B, 100μg/ml PLs+SP-C and 100μg/ml CHF5633 for 4h. Cells were lysed and total RNA was extracted for TNF-α (A), IL-1β (B), IL-8 (C) and IL-10 mRNA (D) quantification by real-time quantitative PCR. LPS-stimulated (100ng/ml) CD14⁺ cells served as positive control. Relative expression is given as mean ± SD of 4 independent experiments (*p < 0.05, **p < 0.01).