Polarization of CD3⁺ lymphocytes by whole tumour-lysate pulsed DCs.

<p>To examine the nature of T-cell responses induced by whole tumour-lysate pulsed DCs, syngeneic naïve T-cells from MMTV-<i>Ras</i> mice were cultured with either unloaded DCs or TAA-DCs for 1-3-5-7- days and both T cell- and DC-derived cytokines were measured. ELISA quantified released type 1 [IFN-γ (A), IL-12 (C)] and type 2 [IL-10 (B)] cytokines in the supernatants. Results are expressed as mean values ± standard error of three independent experiments. *P value < 0.05; **P value <0.005.