Time study of trans-fucosylation catalysed by Thma and Mfuc5.
- Mateusz Lezyk (847370)
- Carsten Jers (356447)
- Louise Kjaerulff (847371)
- Charlotte H. Gotfredsen (847372)
- Maria D. Mikkelsen (847373)
- Jørn D. Mikkelsen (505586)
Publication date
January 2016
DOIAbstract
<p>Reactions were done using 25 mM pNP-Fuc as donor and 100 mM lactose as acceptor at 30°C and optimal pH. The concentrations were determined by HPAEC-PAD and for pNP by spectrophotometry. L-fucose (■), pNP (⧫), pNP-Fuc (X), 2’-FL (▲), and 3-FL (●).</p
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