Constitutive levels of expression of chemokines and genes related to B cell activity in the different skin sections.

<p>The skin sections indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0147477#pone.0147477.g001" target="_blank">Fig 1</a> were sampled from naïve fish for RNA extraction and analysis of immune gene transcription through real time PCR. Data are shown as the mean relative gene expression normalized to the transcription of the house-keeping gene EF-1α ± SD (n = 10). Relative expression values were lowest in section 7 for most genes analyzed and were set as 1 for comparative reasons. Asterisks indicate significant differences (<i>P</i> < 0.05) relative to values in section 7.