Gene profiling of inflammatory response after TBI across time.

<p>qRT-PCR gene profiling of pooled ipsilateral perilesion cortices after injury. (<b>A)</b> Using a custom curated qRT-PCR mini array of 91 genes identified 55, 71, and 59 genes of interest with a fold change greater than 1.5 over sham values for 1d, 2d, and 7d, respectively (n = 8/group). These genes were then grouped using simple-set theory, displayed graphically as a proportional Venn diagram. Therein, there were multiple overlapping nodes between each time point. The greatest degree of commonly expressed genes was shared with the 2d time point between both 1d and 7d. Comparatively, there were relatively few commonly expressed genes between 1 and 7 days. Importantly, across all time points there were 32 commonly expressed genes as response to TBI. (<b>B)</b> Representative heatmap of Log₂ normalized fold changes for the 32 common-node genes. (<b>C)</b> Gene table for all genes in the curated profiling array. Bold items indicate gene name, with their respective RefSeq identifier listed immediately below. Red = upregulated, Blue = downregulated gene expression.