IL-10 reverses TNF-induced monocytes adhesion to endothelial cells.

<p>Endothelial Cells (ECs) interaction with monocytes was determined after inflammatory insult. (<b>A)</b> ECs were grown to confluency and U937 monocytes, labeled with DiI, were seeded on top of EC monolayers and were cultured for 6 h in the presence of TNF, IL-10 or simultaneously with both cytokines. After treatments, non-adherent cells were washed and culture dishes were visualized under fluorescence microscope to count the adherent monocytes. Original magnification of images was 200X. (<b>B)</b> TNF treatment strongly induced the adhesion of monocytes with endothelial cells. IL-10 strongly inhibited the adhesion of monocytes to ECs. N = 4.