<i>Runx1 P2</i> expression in GM-restricted progenitors enriches for monocyte/macrophage specification.

<p>(A) Contour plots of adult BM Lin^- Sca1^- cKit⁺ (LK) GM progenitors. The GMP and PreGM progenitors can be distinguished on the basis of CD41, CD16/32, SLAMF1 (CD150) and Endoglin expression. (B) Representative FACS plots of P1-GFP/P2-hCD4 expression in PreGM and GMP cells. (C–E) CFU-C activity of <i>WT</i>, <i>P1</i>^<i>+</i><i>P2</i>^<i>-</i> and <i>P1</i>^<i>+</i><i>P2</i>^<i>+</i> PreGMs and GMPs following culture in pro-myeloid semi-solid methylcellulose-based medium. (C) Total CFU-C numbers (%). (D-E) Granulocyte (CFU-G), macrophage (CFU-M) and granulocyte/macrophage (CFU-GM) colony forming unit numbers per 100 plated PreGMs (D) or 500 plated GMPs (E). n = 4 independent experiments.