Expression of (P)RR/Atp6ap2 mRNA along the mouse nephron.

<p>Nephron segments were dissected from mouse kidney and relative mRNA abundance of the (P)RR and the B1 (Atp6v1b1) subunit of the vacuolar H⁺-ATPase assessed. Segment-specific enrichment of nephron fragments was tested by qPCR for various transcripts: Podoplanin for the glomerulum, NaPi-.IIa for the S1 /S2, S3 segments of the proximal tubule and DCT, NKCC2 for the TAL, NCC for the DCT, AQP2 for the collecting duct system, and pendrin for the CNT/CCD. Data are mean ± SEM (n = 4 mice/segments). Glom glomerulus, S1/S2 convoluted part of the proximal tubule, S3 straight part of the proximal tubule, thin limb thin descending and ascending limb of the loop of Henle, TAL thick ascending limb of the loop of Henle, DCT distal convoluted tubule, CNT/CCD connecting tubule/cortical collecting duct, OMCD outer medullary collecting duct, IMCD inner medullary collecting duct, WT M 12 wk C57BL/6 male mouse 12 weeks old.