Stau1 overexpression regulates the alternative splicing of numerous ASEs which are both beneficial and detrimental in DM1 conditions.

<p>(A) A total of 33 ASEs were considered for this analysis based on the following criteria. First, ASEs that showed a ≥10% ΔPSI from WT to DM1 conditions were selected. From this, ASEs that showed a ≥10% ΔPSI with Stau1 overexpression were selected and identified as either beneficial or detrimental for the DM1 pathology (refer to results). (B) Bar graphs showing the specific ASEs that the overexpression of Stau1-HA under DM1 conditions either returned towards the WT splicing pattern (left bar graph) or continued towards a detrimental effect (right bar graph). (C-E) Comparison of the data obtained from the RT-PCR splicing screen presented here to that of Klinck et al., 2014. The threshold used by Klinck et al., was applied (≥5% PSI) to compare the 163 ASEs used in both screens (Refer to <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005827#pgen.1005827.s005" target="_blank">S1 Table</a>). (E) The direction of splicing was determined by identifying whether modification of the splicing proteins (i.e. Stau1 and/or MBNL1/RBFOX1) shifted the splicing pattern of an ASE in similar directions, for example, modification of both splicing factors resulted in an increased splicing of the long isoform.