High-resolution immunofluorescence analysis of the subcellular localization of the ciliary ultrastructure markers.

<p>αβ-tubulin (red; in A) or acetylated α-tubulin (green; in B, C) were markers of microtubules; DNAH5 (green, in A) and DNAI2 (green, in B)–of outer dynein arms; DNALI1 (red, in C)–of inner dynein arms; CCDC39 (red, in D)–of nexin-dynein regulatory complex, and LRRC6 (red, in E)–of cytoplasmic assembly complex. DIC, differential interference contrast; nuclei were stained with Hoechst 33342 (blue). Respiratory epithelium cells from the control and from patient #683 are shown in upper and lower panels, respectively. White scale bars represent 10 μm.