Identification of cellular sources of TGFβ in PBMCs of VL patients (n = 10), ECs (n = 5) and NECs (n = 5).

<p>Total PBMCs were freshly cultured in the presence of PMA (50 ng/μl), ionomycin (1 μg/μl) for 2 hrs and for additional 1 hr in presence of brefeldin A (10μg/μl) before staining. (A) Percentages of CD25+ and CD25− cells among CD4+TGFβ+ cells. (B) Percentages of CD25+ and CD25− cells among CD4+FoxP3+TGFβ+ cells. Data are represented as mean ± SE. <i>P</i> values were calculated using Wilcoxon matched pairs signed rank test for paired samples; <i>P</i><0.05 was considered significant. (C), (i)-(iv) Data showing one representative healthy control. (D), (i)-(iv) Data showing one representative active VL patient.