Effect of leptin on MMP mRNA and protein synthesis in HGFs.

<p>HGFs were stimulated with leptin (0–25 μg/ml) for 24 h; <i>MMP-1</i> gene expression was assessed by real-time RT-PCR (A) and supernatant MMP-1 concentrations were assessed by ELISA (B). HGFs were stimulated with leptin (10 μg/ml) for 24 h or the indicated durations and supernatant MMP-1 (C) and MMP-3 (D) concentrations were assessed by ELISA. HGFs were stimulated with leptin (0–25 μg/ml) for 24 h and <i>MMP-14</i> gene expression was assessed by real-time RT-PCR (E). HGFs were stimulated with IL-1α (0.05 ng/ml) or OSM (5 ng/ml) alone, or in combination with leptin (10 μg/ml) (F) and pam2CSK4 (50 ng/ml) or <i>E</i>. <i>coli</i> LPS (10 ng/ml) alone, or in combination with leptin (10 μg/ml) for 24 h (<b>G</b>) and <i>MMP-14</i> gene expression was determined by real-time RT-PCR. RT-PCR data (fold control) are expressed relative to RNAP. Data (excluding Fig 2D) are presented as mean+SEM HGF cultures from 3 HGF donors stimulated in independent experiments (n = 4 from each donor). Remaining data (Fig 2D) are expressed as mean+SD from 3 individual HGF donors (n = 4 from each donor). *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 compared to the unstimulated control at the same time point.