Morphology of hMSCs seeded on PCL platforms fabricated with OpenSLS.

<p>a) When hMSCs, constitutively expressing GFP (cytoplasm) and H2B-mCherry (nucleus), were seeded on tissue culture plastic (TCP), cells exhibited elongated, spindle-like morphology and alignment of neighboring cells. b) Schematic depicting seeding of GFP/H2B-mCherry-labeled hMSCs onto sintered (unsmoothed) as well as vapor-smoothed PCL platforms. c) After 10 days in culture, hMSCs populated the surface of the sintered PCL platform as a sparse monolayer (scale bar = 1000 μm). d) hMSCs grown on sintered PCL exhibit a spindle-like morphology but are not spread out or aligned to the degree observed on TCP (scale bar = 100 μm). e) On sintered, vapor-smoothed PCL, a dense monolayer of hMSCs was observed with regions of local cell alignment (scale bar = 1000 μm). f) In contrast to hMSCs grown on sintered, unsmoothed PCL, those seeded on vapor-smoothed PCL exhibited highly elongated spindle-like morphology characteristic of hMSC culture on TCP (scale bar = 100 μm). Gamma correction was used to improve visualization of cells.